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PurificationotEarthwormThrom bolyticEnzymeandItsEffectson CoagulativeFunctionandFibrin olysis

摘要撰写人 : TsingHua
浏览次数 : 5  词语: 300   出版日期: 六月 20, 1996
PurificationotEarthwormThrombolyticEnzymeand ItsEffectsonCoagulativeFunctionandFibrinoly sisPurificationotEarthwormThrombolyticEnzym eandItsEffectsonCoagulativeFunctionandFibri nolysisCHENGNiu-liang(程牛亮);NIUBo(牛勃);andZHE NGGuo-ping(郑国平),etal(DepsartmentofBiochemis try,ShanxiMedicalffellege,Taiyuan030001)Ino rdertostudythemechanismofearth-wnrrnonpromo tingblcodcirculationtore-moyestasis,andeart hworlnthrombolyticen-zymewasisolatedandpuri fiedfromthecrudeextractoftheearthworintissu ebytheuseofanimoniuinsulfateandcoluinnchrom ato-graphysuccessivelywithDEAE-celluloseand SephacrylS-200columns.Theenzymecoulddissolv etheexperimentalthrombusofrabbit,theclotsof humanbloodandpurifiedfibrin.Theexperimental resultsshowedthattheen-zymecouldsiguificant lyprolongkaolinpartialthrombintime(KPTT),pr othrombintime(PT)andthrombintime(TT),P<0.01 ,lowerthecontentofplasmafibrinogenandin-cre asethefibrin(fibrinogen)degradationproduct, P<0.01.Theresultofthefibri-nolysodynamicogr aniindicatedthattheen-zwnecouldchangethefib rinolysodynanico-graxnofnortnalplasmatolowa ndflatstate.Alloftheseeffectsdemonstratedth attheabili-tyoftheeratiiworrnthrombolyticen zymecouldresistbloodcoagulationandpromotefi b-rinolysis.OrzgznalarticleonCJIM(Chin)1995 ,15(Sup-pl.):86REFERENCES||1.裴德恺,冯秉安.胆结石溶石疗 法的现状与展望.实用外科杂志1991;11(1):18-21.2.管田文夫.胆石治疗新 展开──(1)胆石溶解疗法.日消志1994;91(增刊):805-806.3.冯秉安, 高静涛,裴德恺,等.金石散胶羹治疗胆石症134例临床观察.实用中医杂志1994;10( 1):5-8.4.陈 奇主编.中药药理研究方法学.北京:人民卫生出版社,1993:45 1-454.5.朱培庭,张静哲,王以实,等.胆宁片、胆通、熊去氧胆酸治疗慢性胆道感染、 胆石病的临床疗效对照研究.中国中西医结合外科杂志1995;1(4):205.6.高静涛,王选琛,陈海波,等.金石散溶解胆石(三种剂量)的效应观察.大连医学院学报1994;16(1):46. PurificationotEarthwormThrombolyticEnzymeand ItsEffectsonCoagulativeFunctionandFibrinoly sis@程牛亮,牛勃,郑国平1.裴德恺,冯秉安.胆结石溶石疗法的现状与展望.实用外科杂 志1991;11(1):18-21.2.管田文夫.胆石治疗新展开──(1)胆石溶解疗法 .日消志1994;91(增刊):805-806.3.冯秉安,高静涛,裴德恺,等.金石散 胶羹治疗胆石症134例临床观察.实用中医杂志1994;10(1):5-8.4.陈 奇主 编.中药药理研究方法学.北京:人民卫生出版社,1993:451-454.5.朱培庭,张 静哲,王以实,等.胆宁片、胆通、熊去氧胆酸治疗慢性胆道感染、胆石病的临床疗效对照研究. 中国中西医结合外科杂志1995;1(4):205.6.高静涛,王选琛,陈海波,等.金石散溶解胆石(三种剂量)的效应观察.大连医学院学报1994;16(1):46.

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