NIonoamine neurotransmitters Irmy exacerbatethe brain ischemic injury,nimodipine(Nim)haS龀Drotective dfect wheh was砌b】ted口【t髓siv≤【y tc)itscalcium antagonist and vaS0dilatatory action。工。,whereas the pc堪sible rOle of Nim on monoamine∞ntents was often neglected. In the present studywe observed the effect of Nim on rat EEG and brainmonoamine contents under cerebral ischemia.MATERIALS AND METHoDS Nim was synthesized by Xinhua Phamaceutical Factory,shandong’ China. Sprague-Dawley rats(0, ,l=24,weighing 240±s 39 g) were used. MPF-4 nuoroscencespectrophotometer、柏s made by Hitachi G)rp,Japall. Assessments were carried 0ut in 4 groups: A) shamoperation; B)iSchemia and reperfusion: C) ischemia andreperfusion with Nim 0.75 mg·kg-1;D)ischemia andreperfusi叽with Nim 1.5 mg·kg_。. The f0Ilr-vessel occlusjon and EEG re删irlg weremade【。·3。.Before ligating bilateral comnlon carotid arteriesand at the beginning of reperfusion, the rats 0f C and Dgroups珊injected intraperitd矧ly(ip)with Nh O.375and O.75 rng·kg一。re甲ectivdy. The rats were decapitated after rcperfusion. Thecerebral cortex aIld Kppocampus、Ⅳere stored in liquidnitrog∞. MonoaIllines were measl蒯 by nuorospec胁phDl姗etry. An da协were expressed as j±s and arlalyzed by t·test.RESUL髑 EEG The anIest of the bl∞d supply to thebrain caused a rapid disappearance 0f EEG actiVity.Duri豫recirculation the recovery time for theischemia group was 39±4 min(,l=6),but theamDlitude waS still Severely inhibited at the end 0frecirculation. In treated ratS, ip Nim 0.75 and1.5 mg.kg一。remarkably quickened the recovery 0fEEG changeS to 19±3 min and 17±4 min(竹=6,P<0.01邯the iSchemic ratS),respectively.C0mpared with the shamoperation group,cortex,hippoc锄puS NE,￡IA,5一HT,and 5.HIAA 0f ischemia group all decreasedsignificantly. With ip Nim 0.75 and 1.5 mg·kg一’the contents 0f NE,DA,5.HT,and 5一HIAA incortex and hippoc锄pus all increa差沱d(Tab 1).Tab 1. Effects of nimod量pIne蚰colttc|I and Mplm∞mp_lm咖_饿衄lIne cont蜘融(n薯;/g wet tl鲻吨)曩t雠re呐I bchem‘aand reperrI嵋ton. 一=6 rI憎,j±$·‘P<0.0l vs sh哪0I嘲mtl呲.dP>O.05。‘P
@可君$河南医科大学药理教研室!郑州 450052 中国
@李志刚$河南医科大学药理教研室!郑州 450052 中国
@刘方洲$河南医科大学药理教研室!郑州 450052 中国尼莫地平;;
脑缺血;;
大脑皮质;;
海马;;脑电描记术目的:研究尼莫地平(Nim)对脑 缺血损伤的作用。方法:大鼠脑缺血模型采用四血管结扎法(4-VO),单胺递质测定采用荧光 分光光度法。结果:腹腔注射尼莫地平0.75mg·kg~(-1)和1.5mg·kg~(- 1)能显著改善缺血再灌注损伤的脑电活动,脑电恢复时间可恢复到19±3min和17±4m in(P<0.01),尼莫地平还能明显减轻缺血30min后再灌注1h的单胺递质的降低。结论:Nim对缺血引起损伤的神经有保护作用。1Lazarewicz JW, Pluts R, Salinska E, Puka M. Benifical effect of nimodipine on metabolic and functional disturbances in rabbit hippocampus following complete cerebral ischemia. Stroke 1989; 20:70-7．
2 Pulsinelli WA, Brierley JB. A new model of bilateral hemispheric ischemia in the unanesthetized rat. Stroke 1979; 10:267-72．
3 Peng XQ, KE J. Effects of 3 henbane drugs on acute forebrain ischemi