IntroductionThe herbSambucus chinensis Lindl.is aneffective medicine for treating hepatitis.Accordingto studies in pharmacology experi ment and clinicpractice,ursolic acidinSambucus chinensis Lindl.is the major effective component for anti-hepatitisactivity<1-3>.1Experi ments1.1MaterialsSambucus chinensis Lindl.is grown inYichun,Jiangxi Province,China.The standardsample of ursolic acid is provided by ChinaInstitute of Identification of Drug and BiologicalProducts.1.2InstrumentsHPLC,Beckman-126/168;FTIR,NicoletI MPACT-400;1H NMR and13C NMR,INOVA-500;Mass Spectrometer,Autospec-Ulti ma ETOFEI and Magnet BPI.1.3Experi mental stepsDried herb raw powder ofSambucus chinensisLindl.was extracted by95%ethanol at80℃for1hfor2ti mes.The two extracts were combined andethanol was recycled under vacuum.The resultedslush was treated byi mpurity remover“YCXY-1”,separated by gelation and then decolorized withactive carbon.After purification and final air-drying,colorless needle-like crystalline ursolic acidwas obtained.2Sample identification2.2Thinlayer chromatography2mg ursolic acid was dissolved in1ml95%ethanol,and the sample was applied to hard TLCplate of silica sel G,along with standard sample ascontrast.Mixture of cyclohexane and acetone(3
∶1)was used as developer.After air drying theplates were sprayed with10%sulfuric acid inethanol and heated to110℃for10min to developthe plate.It was found that both the purifiedsample and the standard sample showed the samepurple-red spot under visible light or golden spotunder UVof365nmand the sameRf.2.3Identification of chemical structureThe FTIR,EI-MS,1H NMR,13C NMRspectral data of sample agree with those of ursolicacid reportedinliteratures<4-5>.3Content of ursolic acidConditions for HPLC:Column,C18-ODS150mm×4.6mm,5μm;temperature,roomtemperature;working wavelength,λ=210nm;sensitivity,0.005AUFS;mobile phase,methanol∶water(88∶12);eluting speed,1.0ml.min-1.The sample was dissolved in methanol(2mg.ml-1).20μl of the sample solution was injectedinto the apparatus.The chromatograms werenormalized by external standard method and gavethe ursolic acid content of99.8%.Production process for ursolic acid@李开泉$Yichun College, Yichun 336000, Jiangxi, China
@陈武$Yichun College, Yichun 336000, Jiangxi, China
@李翔一$Yichun College, Yichun 336000, Jiangxi, China<1>Li Kaiquan(李开泉),Chen Wu(陈武),Xiong Xiaojuan(熊筱娟).Chemical research of ursolic acid—the anti-hepatitis component inSambucus chinensis Lindl..Chinese Traditional and Herbal Drugs(中草药),2001,32(suppl.):36-37
<2>Chen Wu(陈武),Li Kaiquan(李开泉),Xiong Xiaojuan(熊筱娟).Research of the anti-hepatitis active component of Sambucus chinensis Lindl..Journal of Nanchang University(南昌大学学报),2001,25(2):165-167
<3>Song Jing(宋京),Zhang Qinglin(张庆林).Developments in research of pharmacology of ursolic acid.Chinese Traditional and Herbal Drugs(中草药),1999,30(suppl.):196-198
<4>Cong Puzhu(丛浦珠).Application of Mass Spectroscopy in Organic Chemistry of Natural Products(质谱学在天然有机化学中的应用).Beijing:Science Press,1987:684-686
<5>Peng Shiqi(彭师奇).Interpretation of Drug Spectroscopy(药物的波谱解析).Beijing:Joint Press of Beijing Medical University and Union Medical College,1998
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